Cartilage Degeneration, Subchondral Mineral and Meniscal Mineral Densities in Hartley and Strain 13 Guinea Pigs



Yubo Sun*, 1, Brian P Scannell 1, Patrick R Honeycutt 1, David R Mauerhan 1, James Norton H2, Edward N Hanley Jr1
1 Department of Orthopedic Surgery, Cannon Research, Carolinas Medical Center, PO Box 32861, Charlotte, NC 28232, USA
2 Dickson Advanced Analytics Group, Carolinas Medical Center, PO Box 32861, Charlotte, NC 28232, USA


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 635
Abstract HTML Views: 387
PDF Downloads: 129
Total Views/Downloads: 1151
Unique Statistics:

Full-Text HTML Views: 356
Abstract HTML Views: 226
PDF Downloads: 98
Total Views/Downloads: 680



© Sun et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Orthopedic Surgery, Cannon Research 303, Carolinas Medical Center, PO Box 32861, Charlotte, NC 28232, USA; Tel: 704/355-5656; Fax: 704/355-5620; E-mail: yubo.sun@carolinashealthcare.org


Abstract

Osteoarthritis is a joint disease involved in articular cartilage, subchondral bone, meniscus and synovial membrane. This study sought to examine cartilage degeneration, subchondral bone mineral density (BMD) and meniscal mineral density (MD) in male Hartley, female Hartley and female strain 13 guinea pigs to determine the association of cartilage degeneration with subchondral BMD and meniscal MD. Cartilage degeneration, subchondral BMD and meniscal MD in 12 months old guinea pigs were examined with histochemistry, X-ray densitometry and calcium analysis. We found that male Hartley guinea pigs had more severe cartilage degeneration, subchondral BMD and meniscal MD than female Hartley guinea pigs, but not female strain 13 guinea pigs. Female strain 13 guinea pigs had more severe cartilage degeneration and higher subchondral BMD, but not meniscal MD, than female Hartley guinea pigs. These findings indicate that higher subchondral BMD, not meniscal MD, is associated with more severe cartilage degeneration in the guinea pigs and suggest that abnormal subchondral BMD may be a therapeutic target for OA treatment. These findings also indicate that the pathogenesis of OA in the male guinea pigs and female guinea pigs are different. Female strain 13 guinea pig may be used to study female gender-specific pathogenesis of OA.

Keywords: Guinea pigs, meniscus, mineral density, osteoarthritis, subchondral bone.