Evaluation of Autoantibodies in Patients with Primary and Secondary Sjogren’s Syndrome

Ellen De Langhe1, Xavier Bossuyt2, Long Shen3, Kishore Malyavantham3, Julian L. Ambrus4, *, Lakshmanan Suresh3, 5
1 Department of Rheumatology, University Hospital Leuven, Herestraat 49, 3000 Leuven, Belgium
2 Laboratory Medicine, University Hospitals Leuven, Belgium and Department of Microbiology and Immunology KU Leuven, Belgium
3 Immco Diagnostics / Trinity Biotech, Buffalo, NY, USA
4 Division of Allergy, Immunology and Rheumatology, SUNY at Buffalo School of Medicine, Buffalo, NY, USA
5 Department of Oral Diagnostic Sciences, SUNY at Buffalo School of Dental Medicine, Buffalo, NY, USA

Article Metrics

CrossRef Citations:
Total Statistics:

Full-Text HTML Views: 5191
Abstract HTML Views: 2254
PDF Downloads: 1103
ePub Downloads: 924
Total Views/Downloads: 9472
Unique Statistics:

Full-Text HTML Views: 2229
Abstract HTML Views: 1265
PDF Downloads: 654
ePub Downloads: 488
Total Views/Downloads: 4636

Creative Commons License
© De Langhe et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Division of Allergy, Immunology and Rheumatology, SUNY at Buffalo School of Medicine, Room C281 Buffalo General Hospital, 100 High Street, Buffalo, NY 14203, USA; Tel: 716-859-2995; Fax: 716-859-1249; E-mail:



Antibodies to salivary gland protein 1 (SP1), carbonic anhydrase 6 (CA6) and parotid secretory protein (PSP) were discovered in an animal model of Sjogren’s syndrome (SS). Their expression was noted in patients with SS, especially those with lower focus scores on lip biopsies and those with early disease lacking antibodies to Ro and La.


The current studies evaluated these autoantibodies in patients with long-standing SS expressing high levels of anti-Ro antibodies and in patients with Sjogren’s syndrome secondary to systemic lupus erythematosus (SLE), systemic sclerosis (SSc) and mixed connective tissue disease (MCTD).


Sera were obtained from patients and evaluated by ELISA for IgG, IgA and IgM antibodies to SP1, CA6 and PSP.


IgA anti-CA6 antibodies were noted in 38% of these patients, but anti-SP1, CA6 and PSP IgM or IgG antibodies were identified only in a minority of patients. In patients with secondary SS, antibodies to SP1/CA6/PSP were more sensitive and specific than anti-Ro .


While more studies are needed, antibodies to SP1, CA6 and PSP provide valuable markers for the diagnosis of primary and secondary SS, especially early in the course of the disease.

Keywords: SLE, MCTD, Systemic Sclerosis, Sjogren’s syndrome.