RESEARCH ARTICLE
Temporal and Spatial Expression Patterns of Bone Morphogenetic Protein 3 in Developing Zebrafish
Midori Ito-Amano 1, Yukio Nakamura*, 1, Mika Morisaki 1, Xinjun He 2, Masanori Hayashi 1, Ramida Watanapokasin 3, Hiroyuki Kato 1
1 Department of Orthopaedic Surgery, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto 390-8621, Japan
2 Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts 02138, USA
3 Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand
Article Information
Identifiers and Pagination:
Year: 2014Volume: 8
First Page: 69
Last Page: 72
Publisher ID: TORJ-8-69
DOI: 10.2174/1874312901408010069
Article History:
Received Date: 4/2/2014Revision Received Date: 18/7/2014
Acceptance Date: 19/7/2014
Electronic publication date: 2 /10/2014
Collection year: 2014
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Abstract
Bone morphogenetic proteins (BMPs) are important elements in bone biology. We herein report the expression profiles of zebrafish bmp3 (zbmp3) as demonstrated by real-time PCR and in situ hybridization. The expression of zbmp3 was highly detectable by real-time PCR from 1 day post-fertilization (1 dpf) to 2 weeks post-fertilization (2 wpf) and peaked at 1 wpf. For in situ hybridization experiments, zbmp3 was expressed in the otic vesicle at 1 dpf, 2 dpf, 3 dpf, and 5 dpf. It was also expressed in the pharyngeal arches, including the opercle, branchiostegal ray, and pectoral fins, at 2 dpf. Our results suggest that zbmp3 may play an important role in the skeletal biology of developing zebrafish.
Keywords: Bmp3, Expression patterns, Zebrafish..
